![]() ![]() The transition to stable binding, followed using 36Cl -, occurred over several minutes. ![]() However, stabilization of Cl -binding could be accomplished by prolonged incubation in the presence of Cl. The Cl -depleted membranes were immediately (<15 s) reactivated to 85−90% of the original activity by the addition of fairly high concentrations of Cl - ( K d = 0.5 mM), but both Cl - and the activity were promptly lost when the membranes immediately after reactivation were diluted in a Cl -free medium. A light-intensity study of the Cl -free membranes showed that all PS II centers were able to evolve oxygen at about 35% of the control rate when measured in Cl -free medium. Photosystem II membranes, dialyzed against a Cl -free buffer to remove bound Cl -, lost about 65% of the control activity. ![]()
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